Journal: eNeuro

Article Title: Intrinsic Cell-Class–Specific Modulation of Intracellular Chloride Levels and Inhibitory Function, in Cortical Networks, between Day and Night

doi: 10.1523/ENEURO.0325-25.2025

Figure Lengend Snippet: Day/night differences in assay of cortical feedforward inhibition, in vivo. Ai , Schematic showing the experimental arrangement of the optic fiber and the multielectrode arrays in the brain slice. Aii , Example traces of local field potentials during a train of stimuli and the derived current source densities. Aiii , Enlarged view of CSDs for the first two stimuli and a color-coded representation of the CSDs, showing that the sink-source sequence is generally found only in a single electrode in the supragranular layers. B , Color-coded representation of each stimulus in a train of 10 (delivered at 10 Hz). C , The peak of the inhibitory (“source”) current for data collected from ZT5 (red) and ZT17 (black) brain slices, showing highly significant differences at the two times, both for the absolute peak values and ( Ci ) the pattern of adaptation from the first pulse in the train ( Cii ).

Article Snippet: A Neuronexus multielectrode probe, with a single recording site on each of 8–16 prongs (100 μm separation of prongs), was inserted into the brain slice at its dorsal pole, oriented orthogonal to the pial surface ( Fig. 5 A ).

Techniques: Inhibition, In Vivo, Slice Preparation, Derivative Assay, Sequencing